Construction of "sleeper" cloning vehicles for enzyme overproduction.

Design and Construction of an Electromagetic Supension System for Vehicles

This paper introduces a novel passive suspension system for ground vehicles. This system is based on a flexible Electromagnetic Shock Absorber (EMSA). In the proposed system, efforts are made to a) select a high damping coefficient usable in a car b) determine Physical dimensions and geometry not much different from those of the mechanical shock absorbers and c) seletct EMSA weight and volume l...

Design and Construction of an Electromagetic Supension System for Vehicles

This paper introduces a novel passive suspension system for ground vehicles. This system is based on a flexible Electromagnetic Shock Absorber (EMSA). In the proposed system, efforts are made to a) select a high damping coefficient usable in a car b) determine Physical dimensions and geometry not much different from those of the mechanical shock absorbers and c) seletct EMSA weight and volume l...

plasmid for overproduction of the enzyme . ( fre ) of Escherichia coli and construction of a

The construction of new vehicles for the cloning of transcription termination signals.

We have constructed new plasmids that can be used to clone transcription terminator containing DNA fragments between the first gene of the tryptophan (trp) operon and the tetracycline resistance (tet) gene. Both genes are under control of the trp promotor. Therefore the presence of a transcription termination signal on cloned fragments can be monitored by a decrease in expression of the tet gen...

Cloning of cDNA for argininosuccinate synthetase mRNA and study of enzyme overproduction in a human cell line.

Previous studies of the human cell line RPMI-2650 (wild type) and its canavanine-resistant variants have demonstrated differences in argininosuccinate synthetase activity as follows: canavanine-resistant much greater than wild type grown in citrulline greater than wild type grown in arginine (Su, T.-S., Beaudet, A. L., and O'Brien, W. E. (1981) Biochemistry 20, 2956-2960). A recombinant plasmid...